News Reports & Publications

Molecular Basis for CesT Recognition of Type III Secretion Effectors in Enteropathogenic Escherichia coli.

In the Coombes lab, Dustin is characterizing novel proteins involved in host-pathogen interactions and studying mechanisms of Type 3 secretion.

Enteropathogenic E. coli (EPEC) are pathogenic bacteria that infect the intestinal tract. They do this by utilizing a needle-like injection apparatus called the type III secretion system (T3SS) to deliver protein effectors into the host.

The translocated intimin receptor (Tir) is the first effector to be delivered and is an essential colonization factor required for infection. Although Tir requires the multi-cargo chaperone CesT to enter the host, little is known about the structural and mechanistic details behind this important interaction.

In a recent publication from Dr. Brian Coombes’ lab, Postdoctoral Fellow Dr. Dustin Little identified a novel CesT binding site on Tir, which is critical for Tir secretion and thus essential for successful host infection.

Read the full publication in PLOS Pathogens.